About Our Team

Manny different profiles working hands in hands to understand and eventually cure epilesy

Stéphane Charpier – Pr

s.charpier@charpierlab.fr


Animal models in vivo

Intracellular & feild electrophysiology

Epilepsy, excitability


Stephane Charpier is a professor of neurosciences at the University Pierre and Marie Curie and the head of this team. He received the PhD degree at the Pasteur Institute in Paris during which he obtained the first direct evidence of long-term potentiation at central inhibitory synapses. Since 2007, his researches mainly focus on the processes of initiation, generalization and remote control of absence seizures. With his colleagues, using a genetic model of this infantile epilepsy, he unveiled the cortical neurons responsible for the triggering of paroxysmal activities, which are secondarily propagated and generalized in the cortico-thalamo-cortical loops. He also demonstrated that these epileptic activities are propagated in the basal ganglia networks, which ultimately send back a desynchronizing signal to the cortex, leading to the resolution of the seizures. These findings were awarded by the « Prix Valérie Chamaillard », which recognizes original discoveries on childhood epilepsy. Using the same animal model and performing electroclinical investigations from epileptic children, he currently explores the brain mechanisms by which this form of epilepsy produces a transient impairment of consciousness. In parallel, and in collaboration with the intensive care unit of the hospital Pitié-Salpêtrière, he coordinates a transversal research program aiming at determining the persistent functional properties in cortical neurons and networks during extreme comatose associated with a complete abolition of brain electrical activity.

Mario Chavez – PhD

m.chavez@charpierlab.fr

Electrophysiological signals

Quantitative approach

Brain signal processing


Mario Chavez has a background (M.S. and Ph.D. degrees) in brain signal processing. After holding different postdoctoral positions in the field of nonlinear physics and biomedical signal processing, he became a researcher at Centre National de la Recherche Scientifique (CNRS). His research activities concern new methodologies for characterising functional connectivity of electrophysiological signals recorded at multiple scales (LFP/MEG/EEG/SEEG/fMRI). He has developed a network-based framework to quantify the functional interactions between different neural structures involved in generation and propagation of epileptic activities.

Michel Le Van Quyen  – PhD

m.lvq@charpierlab.fr

Human investigation

Intracerebral electrodes

Epilepsy, Neurofeedback


Michel Le Van Quyen is an Engineer in Telecommunication (Sup Telecom Bretagne, 1990) and, since 2000, INSERM researcher. His main objective is to develop, on the basis of specific electrophysiological patterns, new methodological approaches to improve human health. For example, in collaboration with 3 European hospitals (projet EPILEPSIAE, FP7), he developed a real-time automated seizure warning and prevention system for use by epileptic patients and their caregivers. Its evaluation first provided a “proof-of-principle” demonstration of the feasibility of a prospective seizure prediction in a significant number of patients. Currently, he is investigating the cellular mechanisms of seizure precursors using micro-electrode recordings from patients with  focal epilepsies. He published 67 papers in international peer reviewed journals  and initiated 3 patents.

Vincent Navarro – MD PhD PU-PH

v.navarro@charpierlab.fr

Human & animal investigations

Intracerebral field & single unit signals

Epilepsy, clinical research


Vincent Navarro is professor of Neurology at Pierre and Marie Curie University (Sorbonne Universities). He is specialized in diagnosis and treatment of epilepsy in human patients, and is the head of the EEG Unit, at the Pitié-Salpêtrière Hospital. His research focus on the pathophysiology of the epilepsies using different approaches such as, in vivo intracranial electrophysiology in Humans (by means of microelectrodes recordings) and in vivo electrophysiology in murine models of epilepsy. In addition, he has major interests in the understanding of status epilepticus and auto-immune epilepsies, mediated by antibodies directed against synaptic proteins.

More on Pr. Navarro's reseach interests

In vivo electrophysiology in Humans: Microelectrodes recording in patients.

I am working on the pathophysiology of the seizure emergence using multi-level recordings. In patients suffering from partial pharmacoresistant epilepsies, intracranial EEG recordings can be performed as a presurgical evaluation in order to identify precisely the epileptogenic focus. Numerous electrodes explore the cortex of several lobes. In addition, we are now using microelectrodes that are recording very small populations of neurons. We are able to record continuously 24 hours per day, during 3-4 weeks, the macro and the microelectrodes (until 160 channels) at high frequency sampling. We are studying the relationship between the macro- and the microelectrodes activities. We are identifying micro-events, detectable only on the microelectrodes: the ‘microseizures’ are ictal events rarely observed on the local field potential (LFP) of the micro-electrodes. We are also analyzing the relationship between the LFP and the multiunit activity (MUA). Spike sorting techniques would identify the long-term behavior of individual neurons with the objectives to describe specific changes during the period preceding the seizures. In vivo recordings from the microelectrodes are also an unique window to explore the neuronal code from cognitive functions.

This project associates numerous competences: electrophysiologists (Claude Adam), neurosurgeons (Stephane Clemenceau, Hayat Belaïd, Bertrand Mathon), neuroradiologists (Dominique Hasboun), and researchers (Michel Le Van Quyen) and engineer (Katia Lehongre).

This program is supported by the IHU and ICM-OCIRP fundings.

 

In vivo electrophysiology in murine models of epilepsy.

Experimental electrophysiology is necessary as a complement of the human recordings to elucidate some mechanisms implied in the pathophysiology of the partial seizures. I have developed a video-EEG unit to record EEG activity from freely moving rat or mice. We are now installing a system to record continuously 24 hour per day, several animals at the same time. Electrodes can be placed in the cortex or in deep structures, like the hippocampus. We can record transgenic mice overexpressing or inactivated for a specific gene. We can also record animal receiving an excitotoxic drug, as the kaïnate acid. The video is acquired simultaneously with the EEG and allow to describe finely the epileptic phenotype of the animals.

This project implies engineers for the experimental tasks (Delphine Roussel and Adrien Schramm), for data processing (Katia Lehongre), and electrophysiologist (Virginie Lambrecq).

This program is supported by the IHU fundings

 

Status epilepticus and auto-immune encephalitis

Status epilepticus are defined as seizures that cannot spontaneously stop. In the case of generalized tonic-clonic status epilepticus, there is a risk of death due to respiratory and hemodynamic failures. In partial status epilepticus, the main risk is the cognitive sequellae, as prolonged and sustained epileptic discharges may cause excitotoxicity and focal neuronal death.  I am involved in therapeutical trials in human patients, in collaboration with the prehospital SAMU physicists. I am also working on patients presenting with hyper-refractory status epilepticus, who need prolonged hospitalization in intensive care units and isoelectric/‘flat’ EEG due to high dose of phenobarbital. There are now growing evidences that these particular status are due to auto-immune encephalitis. Antibodies directed again synaptic proteins have been described. We are now studying the human phenotype associated with specific autoantibodies, trying to identify new auto-antibodies in patients with status epilepticus, and finally we are studying how the antibodies may cause the seizures and the status in animals.

In addition, we are studying new pathway in the lipid homeostasis that may participate to the neuronal death during status.

This project implies collaborations within the French national center for neurological paraneoplasic syndromes (JY Delattre, at the ICM, Paris and J Honnorat at Lyon), the SAMU (Pierre Carli and collaborators), the neurological intensive care unit at the Pitié-Salpêtrière (Sophie Demeret, Francis Bolgert), electrophysiologist (Vi-Huong Nguyen-Michel) and biochemists (Foudil Lamari, Randa Bittar).

Pr. Navarro about epilepsy

Séverine Mahon  – PhD

s.mahon@charpierlab.fr

Animal models in vivo

Intracellular & field electrophysiology

Epilepsy, excitability


Research Staff

Isabel Seror

i.seror@charpierlab.fr

Admintrative tasks

Ordering

Welcoming new members


Isabel is the lab manager, she is there to solve everyday’s issues and drive us through the administrative maze.

Sarah Lecas
s.lecas@charpierlab.fr

Human and animal epileptic tissue

Histology, neuron reconstruction

Epilepsy


Sarah is the histologist of the lab. She studied cellular and molecular biology at Orléans University. After working on cancer and radiation research, she studied biological effects of pollution on skin. During this latter project she learned histology techniques . She joined the Charpier’s team in 2014 using her talents on the brain and to make beautiful neuronal reconstruction.

Delphine Roussel

d.roussel@charpierlab.fr

Animal model in vivo

EMG, video EEG

Epilepsy, parkinson

Delphine Roussel runs the Rodent Neurophysiology in vivo core facility of the institute under Vincent Navarro and Sophie Nicole supervision. Within the preclinical department, the Rodent Neurophysiology in vivo core facility offers its services to perform the neuroelectrophysiological phenotyping of any rodent models of human diseases. This structure is part of  the overall group of facilities of the ICM that aim at favoring translational research projects. Delphine skills includes the standard neurophysiological techniques such as EMG, ENMG, EP. She is also involved in the setting up of a  multi-days continuous video-EEG recording method.

Postdocs

Virginie Lambrecq – MD PhD

v.lambrecq@charpierlab.fr

Human & animal investigations

Intracerebral field & single unit singals

Epilepsy, clinical research


Jimena Ballestero – PhD

j.ballestero@charpierlab.fr

Animal model in vivo

Intracellular & field electrophysiology

Isoelectric coma, excitability


Jimena Ballestero is a Biologist from the University of Buenos Aires (Argentina). She did her PhD in Buenos Aires in Dr. Elgoyhen’s lab where she studied the modulation of cochlear hair cells (the auditory primary receptors) by the olivo-cochlear efferent synapses; the final stage of a feedback system that controls the gain of the auditory input. She then did a fist postdoc at Prof. McAlpine’s group (Ear Institute, UCL, London, UK) studying the electrical properties of the MSO and LSO principal neurons (the first stage of binaural integration on the ascending auditory system) with the aim of understanding their processing capabilities of at the cellular level. In 2016 she joined Charpier’s lab where she is working in understanding the neuronal and network activity underlying deep comatose states. Her approach consists of combining in-vivo intracellular and ECoG recordings in anesthetized rats and EEG and EP recordings on epileptic patients in collaboration with Dr. V Navarro.

Adrien Schramm – PhD

a.schramm@charpierlab.fr

Animal model in vivo

Intracellular & field electrophysiology

Epilepsy, excitability


Adrien Schramm did his PhD with Lyle Graham focusing on the role of shunting inhibition and the BK current in computation at the single cell level and in the context of processing visual information in the cortex. For this he used the dynamic clamp technique to apply conductances to neurons in the whole cell patch configuration in vivo. He then did a first postdoc in Prakash Kara’s lab in MUSC (South Carolina, USA) studying at the subcellular level the functional rules of synaptic circuitry giving rise to different cortical maps in the visual cortex. There he learned how to combine intracellular electrophysiology to two photon microscopy imaging in vivo. He came back to France in Stephane Charpier’s lab to work on the cellular basis of epileptic behavior in different models of epilepsy using single cell and field potential approaches.

Graduate Student

Mark Williams

m.williams@charpierlab.fr

Animal model in vivo

Intracellular & field electrophysiology

Epilepsy, excitability


Aurélie Hanin

a.hanin@charpierlab.fr

Human & animal investigations

Epilepsy, clinical research

Biomarkers


Aurélie Hanin  is a pharmacist from the University of René Descartes (Paris). She joined the lab in 2016 as Master Student in Pharmacology under Dr. Vincent Navarro’s supervision. Her project aimed to describe new biomarkers of status epilepticus (SE). She is currently doing a PhD with Dr. Navarro on the identification of valid biomarkers of brain injury in refractory SE. Her experimental approach consists of doing biochemical analysis (cholesterol homeostasis profiling, cholesterol-related proteins, non-lipid markers profiling) on biological samples from human patients (in refractory SE and in control patients) and on samples from mice models of SE.

Under graduate students

 

Affiliated to the lab somehow

 

 

Line Bedos Ulvin – MD
l.bu@charpierlab.fr

Human & animal investigations

Intracerebral field & MUA electrodes

Epilepsy, clinical reasearch

Lab alumni

 

This list is far from accurate.
Alumnus please contact us to add your name or for corrections.

  • M. Williams (2014 – ). Title: “Integration and dynamic processing of sensory information in thalamo-cortical circuits during absence seizures: An in vivo electrophysiological study in a genetic model.” UPMC-Paris 6.
  • M Navarette (2013 – ), Title: Controling cortical oscillations by sensory stimulation Co-direction with M. Valderrama, Universidad de Los Andes, Bogotá, Colombia.
  • J Bagdasaryan (2012-2015), Title: Cognitive control of intracranial activities in epileptic patients”. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6.  Financial support: Studienstiftung des Deutschen Volkes (German National Academic Foundation). Current situation: Postodoctoral fellow at the Brain Research Institute, University of California (USA).
  • T. Altwegg-Boussac (2011–2015). Title: « Functional role of endogneous electrical brain activities: an in vivo intracellular investigation in the neocortex. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6
  • C Alvarado-Rojas (2010-2014), Title: “Prédiction des crises d’épilepsie par les oscillations hautes-fréquences”. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Financial support: EU project Epilepsiae, and Corporation pour les Etudes en France (CEF). Current situation: Assistant professor (Pontificia Universidad Javeriana, Bogotá, Colombia).
  • M. Chipaux (MD) (2009-2012). Title: Functional properties of cortical networks of human and animal with absence-epilepsy: In vivo electrophysiological studies. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Current situation: Neuro-pediatrician (Rothschild Hospital, Paris).
  • M. Pidoux (2008-2012). Title: « Sensory information processing and sensotimotr intercations in the corticostriatal pathway ». Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Current situation: Post-doctoral fellow in J H. Goldberg’s lab (Cornell University).
  • Pierre Olivier Polack – left the lab in 2008. Current situation: Assitant Professor in Rütgers Univ. (NJ, USA)
  • Jeanne Paz – left the lab in 2007 . Current situation: Assitant Professor at Stanford Univ. (CA, USA)
  • Sean Slaght – left the lab in 2002. Current situation: Neurologist and Epileptologist (Hampshire, UK)
  • A. Bellahcen (2015-2016). M2, Dual master in Brain and Mind Sciences, UPMC/Ecole Normale. Subject: “Implication of high frequency oscillations (> 200 Hz) in the emergence of absence-epilepsy seizures: a multi-scale study in vivo in a genetic model”.
  • A. Hanin (2016). M2 Pharmacologie intégrée préclinique et clinique (Université Paris Descartes). Subject : “Biomarker in the epileptic ‘état de mal’ ”
  •  F. Amzallag (2016). M1 intenship (UPMC) at the “Département Universitaire d’Enseignement et de Formation en Orthophonie”. Subject “Functional cartography of visual areas”
  •  V. Roubeau (2015). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject : “Caractéristiques cliniques, électroencéphalographiques et immunohistochimiques d’un modèle murin d’encéphalite auto-immune”
  •  R. Cuperman Coifman (2015) Master internship inBiomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). “Automatic epileptic seizure detection in EEG based on convolutional neural networks”.
  •  D. Rimsky-Robert (2014-2015). M2 CogMaster-UPMC Paris 6.  Subject “Volontary control of high-frequency oscillations (30-80 Hz) in the human cortex”.
  •  A. Sanchez (2014).Master internship inBiomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). Subject: “Close-loop training of attention using a low-cost EEG device”.
  •  V. Roa-Brith (2014). M1 Master BIP-UPMC Paris 6. “Neurofeedback of slow cortical potentials”.
  •  L. Bedos Ulvin (2013). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject : “Approche multiniveaux de l’émergence des crises chez 21 patients épileptiques”
  •  B. Tchangang (2013). Master internship in Biomedical Engineering (Institut Supérieur des Bio-Sciences, Université Paris Est-Créteil). Project: “GUI for the spectral analysis of ECoG and intracellular recordings”
  •  M. Quiquempoix (2012). M1 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Impact of background synaptic activity on the excitability of somatosensory cortical neurons in vivo”.
  •  D. Luis (2013). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject. Subjecvt : “Modification de l’homéostasie du cholestérol dans les états de mal épileptiques chez l’homme et l’animal”
  •  E. Rullier & R. Kambou (2016-2017). Subject: «Initiation to the scientific research: how to study epilepsy using animal models”  Chercheurs en herbe Program.
  • V. Zapata (2016-2017). Engineering internship in Biomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). Subject: “Control of sleep oscillations by auditory close-loop stimulation”.
  • J. Victor (2016). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Functional properties of cortical neurons and networks during and following prolonged asphyxia”.
  • I. Leroy (2016). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Electrophysiological properties of cortical neurons initiating absence seizures in a rodent genetic model”.
  • E. Soulier (2016). Engineering internship (Ecole des Ponts Paris Tech). Subject: “Boosting brain oscillations during sleep by sensory stimulations: a multi-scale and translational study in humans and rodents”.
  • R. Deudon (2015). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Excitability and transfer function of cortical neurons in a genetic model of familial epilepsy”.
  • A. Zagala (2015). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Optimisation of an in vivo electrophysiology set-up”.
  • Andrea Sanchez (2015)
  • R. Charles, H. Taha, H. Galatée, D. Hung-Cuong,  Z. Weihan (2015-2016). Stage PSC HSS05 de l’Ecole Polytechnique.
  • Q. Soulet de Brugière, H. Mercier (2014) Engineering internship (“technologies et entrepreneuriat”). Ecole Polytechnique.
  • R. Achargui (2014). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Optimisation of an electrophysiology set-up and assessment of cortical neurons excitability in vivo”.
  • M. Druart (2014). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Caracterisation of a new genetic model of familial epilepsy, the DEPDC5 rat”.
  • Alexandra Urbano (2014)
  • T. de Saint-Viance (2014). L1 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “How to characterize the basic electrophysiological properties of pyramidal cortical neurons in vivo”.
  • P. Bruneau (2013). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Cortical rhythms and neuronal excitability: an intracellular in vivo study”.
  • Michael Quicampoix (2012)
  • Boris-Emmanuel Tchangang (2012)
  • Takafumi Arakaki (2012)