Stéphane Charpier – Pr

Animal models in vivo

Intracellular & feild electrophysiology

Epilepsy, excitability

Stephane Charpier is a professor of neurosciences at the University Pierre and Marie Curie and the head of this team. He received the PhD degree at the Pasteur Institute in Paris during which he obtained the first direct evidence of long-term potentiation at central inhibitory synapses. Since 2007, his researches mainly focus on the processes of initiation, generalization and remote control of absence seizures. With his colleagues, using a genetic model of this infantile epilepsy, he unveiled the cortical neurons responsible for the triggering of paroxysmal activities, which are secondarily propagated and generalized in the cortico-thalamo-cortical loops. He also demonstrated that these epileptic activities are propagated in the basal ganglia networks, which ultimately send back a desynchronizing signal to the cortex, leading to the resolution of the seizures. These findings were awarded by the « Prix Valérie Chamaillard », which recognizes original discoveries on childhood epilepsy. Using the same animal model and performing electroclinical investigations from epileptic children, he currently explores the brain mechanisms by which this form of epilepsy produces a transient impairment of consciousness. In parallel, and in collaboration with the intensive care unit of the hospital Pitié-Salpêtrière, he coordinates a transversal research program aiming at determining the persistent functional properties in cortical neurons and networks during extreme comatose associated with a complete abolition of brain electrical activity.

Mario Chavez – PhD

Electrophysiological signals

Quantitative approach

Brain signal processing

Mario Chavez has a background (M.S. and Ph.D. degrees) in brain signal processing. After holding different postdoctoral positions in the field of nonlinear physics and biomedical signal processing, he became a researcher at Centre National de la Recherche Scientifique (CNRS). His research activities concern new methodologies for characterising functional connectivity of electrophysiological signals recorded at multiple scales (LFP/MEG/EEG/SEEG/fMRI). He has developed a network-based framework to quantify the functional interactions between different neural structures involved in generation and propagation of epileptic activities.

Vincent Navarro – MD PhD PU-PH

Human & animal investigations

Intracerebral field & single unit signals

Epilepsy, clinical research

Vincent Navarro is professor of Neurology at Pierre and Marie Curie University (Sorbonne Universities). He is specialized in diagnosis and treatment of epilepsy in human patients, and is the head of the EEG Unit, at the Pitié-Salpêtrière Hospital. His research focus on the pathophysiology of the epilepsies using different approaches such as, in vivo intracranial electrophysiology in Humans (by means of microelectrodes recordings) and in vivo electrophysiology in murine models of epilepsy. In addition, he has major interests in the understanding of status epilepticus and auto-immune epilepsies, mediated by antibodies directed against synaptic proteins.

More on Pr. Navarro's reseach interests

In vivo electrophysiology in Humans: Microelectrodes recording in patients.

I am working on the pathophysiology of the seizure emergence using multi-level recordings. In patients suffering from partial pharmacoresistant epilepsies, intracranial EEG recordings can be performed as a presurgical evaluation in order to identify precisely the epileptogenic focus. Numerous electrodes explore the cortex of several lobes. In addition, we are now using microelectrodes that are recording very small populations of neurons. We are able to record continuously 24 hours per day, during 3-4 weeks, the macro and the microelectrodes (until 160 channels) at high frequency sampling. We are studying the relationship between the macro- and the microelectrodes activities. We are identifying micro-events, detectable only on the microelectrodes: the ‘microseizures’ are ictal events rarely observed on the local field potential (LFP) of the micro-electrodes. We are also analyzing the relationship between the LFP and the multiunit activity (MUA). Spike sorting techniques would identify the long-term behavior of individual neurons with the objectives to describe specific changes during the period preceding the seizures. In vivo recordings from the microelectrodes are also an unique window to explore the neuronal code from cognitive functions.

This project associates numerous competences: electrophysiologists (Claude Adam), neurosurgeons (Stephane Clemenceau, Hayat Belaïd, Bertrand Mathon), neuroradiologists (Dominique Hasboun), and researchers (Michel Le Van Quyen) and engineer (Katia Lehongre).

This program is supported by the IHU and ICM-OCIRP fundings.


In vivo electrophysiology in murine models of epilepsy.

Experimental electrophysiology is necessary as a complement of the human recordings to elucidate some mechanisms implied in the pathophysiology of the partial seizures. I have developed a video-EEG unit to record EEG activity from freely moving rat or mice. We are now installing a system to record continuously 24 hour per day, several animals at the same time. Electrodes can be placed in the cortex or in deep structures, like the hippocampus. We can record transgenic mice overexpressing or inactivated for a specific gene. We can also record animal receiving an excitotoxic drug, as the kaïnate acid. The video is acquired simultaneously with the EEG and allow to describe finely the epileptic phenotype of the animals.

This project implies engineers for the experimental tasks (Delphine Roussel and Adrien Schramm), for data processing (Katia Lehongre), and electrophysiologist (Virginie Lambrecq).

This program is supported by the IHU fundings


Status epilepticus and auto-immune encephalitis

Status epilepticus are defined as seizures that cannot spontaneously stop. In the case of generalized tonic-clonic status epilepticus, there is a risk of death due to respiratory and hemodynamic failures. In partial status epilepticus, the main risk is the cognitive sequellae, as prolonged and sustained epileptic discharges may cause excitotoxicity and focal neuronal death.  I am involved in therapeutical trials in human patients, in collaboration with the prehospital SAMU physicists. I am also working on patients presenting with hyper-refractory status epilepticus, who need prolonged hospitalization in intensive care units and isoelectric/‘flat’ EEG due to high dose of phenobarbital. There are now growing evidences that these particular status are due to auto-immune encephalitis. Antibodies directed again synaptic proteins have been described. We are now studying the human phenotype associated with specific autoantibodies, trying to identify new auto-antibodies in patients with status epilepticus, and finally we are studying how the antibodies may cause the seizures and the status in animals.

In addition, we are studying new pathway in the lipid homeostasis that may participate to the neuronal death during status.

This project implies collaborations within the French national center for neurological paraneoplasic syndromes (JY Delattre, at the ICM, Paris and J Honnorat at Lyon), the SAMU (Pierre Carli and collaborators), the neurological intensive care unit at the Pitié-Salpêtrière (Sophie Demeret, Francis Bolgert), electrophysiologist (Vi-Huong Nguyen-Michel) and biochemists (Foudil Lamari, Randa Bittar).

Pr. Navarro about epilepsy

Séverine Mahon  – PhD

Animal models in vivo

Intracellular & field electrophysiology

Epilepsy, excitability

Research Staff

Admintrative tasks


Welcoming new members

Mariange is the lab manager, she is there to solve everyday’s issues and drive us through the administrative maze.

Human and animal epileptic tissue

Histology, neuron reconstruction


Sarah is the histologist of the lab. She studied cellular and molecular biology at Orléans University. After working on cancer and radiation research, she studied biological effects of pollution on skin. During this latter project she learned histology techniques . She joined the Charpier’s team in 2014 using her talents on the brain and to make beautiful neuronal reconstruction.

Delphine Roussel

Animal model in vivo

EMG, video EEG

Epilepsy, parkinson

Delphine Roussel runs the Rodent Neurophysiology in vivo core facility of the institute under Vincent Navarro and Sophie Nicole supervision. Within the preclinical department, the Rodent Neurophysiology in vivo core facility offers its services to perform the neuroelectrophysiological phenotyping of any rodent models of human diseases. This structure is part of  the overall group of facilities of the ICM that aim at favoring translational research projects. Delphine skills includes the standard neurophysiological techniques such as EMG, ENMG, EP. She is also involved in the setting up of a  multi-days continuous video-EEG recording method.

Virginie Lambrecq – MD PhD

Human & animal investigations

Intracerebral field & single unit singals

Epilepsy, clinical research


Stephen Whitmarsh – PhD



Art and Brain-Computer-Music-Interfaces

Stephen Whitmarsh hold a MSc in psychology from the University of Amsterdam, a PhD in neuroscience from the Donders Institute for Cognitive Neuroimaging, in Nijmegen, and an MA in Art and Media Production from Linköping University, Sweden. After his PhD, he helped start up the Swedish National Magnetoencephalography facility at Karolinska Institute. He then moved to Paris to work in the Visual Cognition group at École Normale Supérieure with Catherine Tallon-Baudry, until joining us. He is studying ictal and inter-ictal neuronal firing patterns in focal epilepsy, as well as in response to intracranial electrical brain stimulation using human recordings from intracranial (micro)electrodes.

Stephen is also much engaged in interdisciplinary research, outreach and open citizen-science, and for this purpose has developed the EEGsynth, an open-source platform for brain-computer-music-interfaces (BCMI). He is member of the art organization Vision Forum, co-founder of the artistic research collective OuUnPo, as well as the interdisciplinary collective 1+1=3, wherein they develop workshops, visual performances and produce contemporary sound experiments using the EEGsynth.

Valerio Frazzini – MD, PhD

Human Epilepsy

Epilepsy Pathophysiology

Intracranial EEG and Single Unit signals

Valerio Frazzini is a neurologist specialized in the epilepsy field. He works in the Epilepsy and the EEG Unit at the Pitié-Salpêtrière Hospital and is PostDoctoral fellow at the ICM in Epimicro group under Vincent Navarro supervision. His research is mainly focused on the neurophysiological basis of the interictal epileptic activity in patients implanted with intracerebral microlectrodes. His approach points to a multi-level analysis of intracranial EEG, taking together multiple physiological features from the standard electrode signals up to single units activities. During his PhD, his research was mainly focused on the electrophysiological characterization of the aberrant features of the Kv2.1 potassium channels in hippocampal neurons from a genetic mouse model of Alzheimer disease. He was also interested in synaptic plasticity mechanisms and BDNF signaling.

Graduate Student

Human investigation

Intracranial EEG and Single Unit signals

Epilepsy, clinical research

Louis Cousyn is a neurologist specialized in epilepsy at the Pitié-Salpêtrière Hospital. He holds an MSc in neurosciences from Sorbonne Université. He joined the lab in 2018. Laureate of the Poste d’accueil INSERM, he is currently doing a PhD with Vincent Navarro and Mario Chavez on the multilevel preictal changes of functional connectivity during ictogenesis in patients with drug-resistant epilepsy. His approach consists of assessing connectivity metrics in intracranial EEG signals from both macro (local field potentials) and microelectrodes (local field potentials, multiunit activity and single unit activity). He takes into account the different states of vigilance (sleep stages, wakefulness) – which could physiologically modify the connectivity values. He also developed a resting-state protocol to better control the environment, the state of vigilance and the quality of data while recording.

Aurélie Hanin

Human & animal investigations

Epilepsy, clinical research


Aurélie Hanin  is a pharmacist from the University of René Descartes (Paris). She joined the lab in 2016 as Master Student in Pharmacology under Dr. Vincent Navarro’s supervision. Her project aimed to describe new biomarkers of status epilepticus (SE). She is currently doing a PhD with Dr. Navarro on the identification of valid biomarkers of brain injury in refractory SE. Her experimental approach consists of doing biochemical analysis (cholesterol homeostasis profiling, cholesterol-related proteins, non-lipid markers profiling) on biological samples from human patients (in refractory SE and in control patients) and on samples from mice models of SE.

Antoine Carton-Leclercq

Animal model in vivo

Intracellular & field electrophysiology

Isoelectric coma, excitability

Antoine Carton-Leclercq is a PhD Student from Sorbonne University. He joined the lab in 2017 as a Master student under the supervision of Stéphane Charpier and Séverine Mahon. His project aimed at designing a new model of isoelectric coma in the rat and studying the impact of a deep comatose state on neuronal and synaptic functionality. He is currently doing a PhD with Stéphane Charpier to further characterize the changes in excitability and synaptic functionality brought by the isoelectric brain state in its various etiologies, he also aims at testing the possible persistence of plastic properties of neurons and networks in extreme brain states. To achieve this, Antoine uses in vivo intracellular and field electrophysiology in anesthetized and hypothermic rats in addition to EEG and EP recordings in comatose patients in collaboration with Pr. Vincent Navarro.

Animal model in vivo

Epilepsy Pathophysiology


Paul Baudin  is a double-cursus medical-scientist student, laureate of the Ecole de l’INSERM Liliane Bettencourt in 2015. He joined the lab in 2017 as Master Student in Neurophysiology under Pr. Vincent Navarro’s supervision. His project aimed to develop a new animal model of autoimmune encephalitis. He is currently doing a PhD with Pr. Navarro on the pathophysiology of autoimmune encephalitis linked to anti-LGI1 antibodies, in order to better understand the mechanisms leading to epileptogenesis in patients. His experimental approach consists of doing electrophysiological recordings in vivo (EEG, LFP, extracellular and intracellular single cell recording), on different new models of the disease.

Animal model in vivo

Intracellular and field electrophysiology

Transcranial Magnetic Stimulation

Manon Boyer is a PhD Student at Sorbonne University. She joined the lab in 2016 as a Master student under the supervision of Stéphane Charpier and Séverine Mahon. Her project aimed at clarifying what are the neuronal and synaptic underpinnings of non-invasive repeated Transcranial Magnetic Stimulation (rTMS). She is currently doing a PhD under Pr. Rachel Sherrard’s supervision (R2N, IBPS) in collaboration with Pr. Charpier to further characterise the changes in excitability and synaptic function during low intensity magnetic stimulation (LI-rTMS). To understand its effects, Manon uses in vivo intracellular and electrocorticographic recordings in anesthetized rats.

Under graduate students


Machine learning

Signal processing

Electrical engineering

Remy Ben Messaoud hold a Msc in biomedical engineering at Imperial College of London and is now fulfilling his degree of Electrical engineering and computer science of the engineering school CentraleSupélec in Paris Area. He is currently doing his 6 month end-of-study internship in the laboratory under the supervision of Mario Chavez. His project falls within the field of epileptic seizures prediction.  He is studying signal processing and machine learning techniques and their feasibility in the context of Brain Machine Interfaces.  He is mainly working on scalp electro-encephalogram ( EEG ) data of epileptic humans.

Affiliated to the lab somehow



Line Bedos Ulvin – MD

Human & animal investigations

Intracerebral field & MUA electrodes

Epilepsy, clinical reasearch

Vera Dinkelacker – MD-PhD


Human investigations

Network connectivity

Epilepsy, clinical reasearch

Vera Dinkelacker is an epileptologist and neuroscientist with a background in neuroimaging and neurophysiology. Her main research interest is the analysis of network pathology in focal epilepsy.
Vera has received scientific training in Germany and France, with an MD-thesis in cellular neurophysiology in the Max-Planck Göttingen (Prof. Neher, Nobel Prize laureate), a neuroimaging post-doc in Bonn (Prof. Elger) and a PhD in Neuroscience in the ICM (Profs. Dupont and Lehéricy, University Pierre et Marie Curie). In her PhD, she examined structural network alterations in temporal lobe epilepsy and their relation to cognition.
She developed her research focus with the fusion of structural connectivity data, explored with diffusion weighted imaging, and clinical measures of network pathology, such as cognitive dysfunction and epileptic discharge. Based on her description of the interdependence of structural, epileptic and cognitive networks, she obtained a 3 years grant financed by the John Bost Foundation to explore the impact of seizures on cerebral connectivity in childhood epilepsy.
The study EPITRACT probes the structural connectivity in children aged 2 to 15 years, who undergo presurgical evaluation in the Rothschild Foundation. For these children with focal epilepsy, seizure onset and propagation zones are determined with intracerebral recordings. The project, in collaboration with Mario Chavez and Olivier David (Grenoble), aims at scrutinising the impact of the epileptic discharge on local and global brain circuitry in the beginning of the disease.

Lab alumni


This list is far from accurate.
Alumnus please contact us to add your name or for corrections.

  • M. Williams (2014 – 2018 ). Title: “Integration and dynamic processing of sensory information in thalamo-cortical circuits during absence seizures: An in vivo electrophysiological study in a genetic model.” UPMC-Paris 6.
  • M Navarette (2013 – ), Title: Controling cortical oscillations by sensory stimulation Co-direction with M. Valderrama, Universidad de Los Andes, Bogotá, Colombia.
  • J Bagdasaryan (2012-2015), Title: Cognitive control of intracranial activities in epileptic patients”. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6.  Financial support: Studienstiftung des Deutschen Volkes (German National Academic Foundation). Current situation: Postodoctoral fellow at the Brain Research Institute, University of California (USA).
  • T. Altwegg-Boussac (2011–2015). Title: « Functional role of endogneous electrical brain activities: an in vivo intracellular investigation in the neocortex. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6
  • C Alvarado-Rojas (2010-2014), Title: “Prédiction des crises d’épilepsie par les oscillations hautes-fréquences”. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Financial support: EU project Epilepsiae, and Corporation pour les Etudes en France (CEF). Current situation: Assistant professor (Pontificia Universidad Javeriana, Bogotá, Colombia).
  • M. Chipaux (MD) (2009-2012). Title: Functional properties of cortical networks of human and animal with absence-epilepsy: In vivo electrophysiological studies. Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Current situation: Neuro-pediatrician (Rothschild Hospital, Paris).
  • M. Pidoux (2008-2012). Title: « Sensory information processing and sensotimotr intercations in the corticostriatal pathway ». Ecole doctorale Cerveau-Cognition-Comportement–UPMC-Paris 6. Current situation: Post-doctoral fellow in J H. Goldberg’s lab (Cornell University).
  • Pierre Olivier Polack – left the lab in 2008. Current situation: Assitant Professor in Rütgers Univ. (NJ, USA)
  • Jeanne Paz – left the lab in 2007 . Current situation: Assitant Professor at Stanford Univ. (CA, USA)
  • Sean Slaght – left the lab in 2002. Current situation: Neurologist and Epileptologist (Hampshire, UK)
  • A. Bellahcen (2015-2016). M2, Dual master in Brain and Mind Sciences, UPMC/Ecole Normale. Subject: “Implication of high frequency oscillations (> 200 Hz) in the emergence of absence-epilepsy seizures: a multi-scale study in vivo in a genetic model”.
  • A. Hanin (2016). M2 Pharmacologie intégrée préclinique et clinique (Université Paris Descartes). Subject : “Biomarker in the epileptic ‘état de mal’ ”
  •  F. Amzallag (2016). M1 intenship (UPMC) at the “Département Universitaire d’Enseignement et de Formation en Orthophonie”. Subject “Functional cartography of visual areas”
  •  V. Roubeau (2015). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject : “Caractéristiques cliniques, électroencéphalographiques et immunohistochimiques d’un modèle murin d’encéphalite auto-immune”
  •  R. Cuperman Coifman (2015) Master internship inBiomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). “Automatic epileptic seizure detection in EEG based on convolutional neural networks”.
  •  D. Rimsky-Robert (2014-2015). M2 CogMaster-UPMC Paris 6.  Subject “Volontary control of high-frequency oscillations (30-80 Hz) in the human cortex”.
  •  A. Sanchez (2014).Master internship inBiomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). Subject: “Close-loop training of attention using a low-cost EEG device”.
  •  V. Roa-Brith (2014). M1 Master BIP-UPMC Paris 6. “Neurofeedback of slow cortical potentials”.
  •  L. Bedos Ulvin (2013). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject : “Approche multiniveaux de l’émergence des crises chez 21 patients épileptiques”
  •  B. Tchangang (2013). Master internship in Biomedical Engineering (Institut Supérieur des Bio-Sciences, Université Paris Est-Créteil). Project: “GUI for the spectral analysis of ECoG and intracellular recordings”
  •  M. Quiquempoix (2012). M1 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Impact of background synaptic activity on the excitability of somatosensory cortical neurons in vivo”.
  •  D. Luis (2013). M2 in “Biologie Intégrative Spécialité Neurosciences” (Université Pierre et Marie Curie). Subject. Subjecvt : “Modification de l’homéostasie du cholestérol dans les états de mal épileptiques chez l’homme et l’animal”
  •  E. Rullier & R. Kambou (2016-2017). Subject: «Initiation to the scientific research: how to study epilepsy using animal models”  Chercheurs en herbe Program.
  • V. Zapata (2016-2017). Engineering internship in Biomedical Engineering (Universidad de Los Andes, Bogotá, Colombia). Subject: “Control of sleep oscillations by auditory close-loop stimulation”.
  • J. Victor (2016). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Functional properties of cortical neurons and networks during and following prolonged asphyxia”.
  • I. Leroy (2016). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Electrophysiological properties of cortical neurons initiating absence seizures in a rodent genetic model”.
  • E. Soulier (2016). Engineering internship (Ecole des Ponts Paris Tech). Subject: “Boosting brain oscillations during sleep by sensory stimulations: a multi-scale and translational study in humans and rodents”.
  • R. Deudon (2015). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Excitability and transfer function of cortical neurons in a genetic model of familial epilepsy”.
  • A. Zagala (2015). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Optimisation of an in vivo electrophysiology set-up”.
  • Andrea Sanchez (2015)
  • R. Charles, H. Taha, H. Galatée, D. Hung-Cuong,  Z. Weihan (2015-2016). Stage PSC HSS05 de l’Ecole Polytechnique.
  • Q. Soulet de Brugière, H. Mercier (2014) Engineering internship (“technologies et entrepreneuriat”). Ecole Polytechnique.
  • R. Achargui (2014). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Optimisation of an electrophysiology set-up and assessment of cortical neurons excitability in vivo”.
  • M. Druart (2014). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Caracterisation of a new genetic model of familial epilepsy, the DEPDC5 rat”.
  • Alexandra Urbano (2014)
  • T. de Saint-Viance (2014). L1 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “How to characterize the basic electrophysiological properties of pyramidal cortical neurons in vivo”.
  • P. Bruneau (2013). L3 internship in Neuroscience (Université Pierre et Marie Curie). Subject: “Cortical rhythms and neuronal excitability: an intracellular in vivo study”.
  • Michael Quicampoix (2012)
  • Boris-Emmanuel Tchangang (2012)
  • Takafumi Arakaki (2012)