Open positions

Postdoc positions

Neurophysiology, excitability, translational reseach

 

DURATION 18 months (multiple renewal possible)
STARTING DATE Flexible
BRIEF DESCRIPTION OF THE PROJECT Based on new data obtained in the lab, neurons in an iso-electric brain (i.e., without spontaneous activity as occurring during deep coma) keep a remarkable functional and intrinsic excitability. The project aims at describing this excitability and redefine neural state giving rise to specific comas. The primary task will be to take over the duties of the graduate student at the origin of the project, including record neuronal functional responses in anesthetized (intracellular, ECoG) and behaving (LFP) rats as well as in human patients (EEG).

One main objective of this translational study is to link the results in human patients and rat models, therefore excellent relational skills are essential to collaborate with neuroscientists in the lab and neurologists at the hospital.

Specific training will be provided, but electrophysiological expertise is required. Candidates should be highly motivated, meticulous, have good skills in quantitative analysis and be ready to participate in the overall scientific life of the lab. Experience in performing experiments in vivo is a plus.

 

Qualification & experience required PhD in neuroscience, MD with experience in single cell electrophysiology, or physicist with similar background.
Profile, skills & ability required Single cell and field electrophysiological recordings, experience with rodent handling and in vivo experiments, good relational skills, high motivation.

 

Contact: Stéphane Charpier

 

Neurophysiology, epilepsy, translational reseach

 

DURATION 2 years
STARTING DATE 02/01/2019
BRIEF DESCRIPTION OF THE PROJECT 

Multi-scale in vivo electrophysiology and morphology in a rodent genetic model of absence epilepsy

Absence epilepsy is a prototypic form of idiopathic generalized epilepsies whose epileptogenic processes remain largely unknown. The proposed postdoctoral work will consist in exploring in the GAERS, a validated model of the human pathology, the post-natal mechanisms responsible for the building-up of epileptic networks. The intake team recently showed that epileptic discharges first appear in the somatosensory cortex at the beginning of the third post-natal week and progressively evolved, in a three-step maturation process, into full-blown spike-and-wave discharges.

The goal of these post-doctoral researches is to establish causality links between the maturation of epileptic discharges and the post-natal developmental changes in the intrinsic excitability, synaptic function and morphology of the cortical neurons (and associated networks) responsible for the initiation of seizures.

Main activities
  • Combined in vivo EEG and intracellular (or patch-clamp) recordings from somatosensory cortex neurons in awake (or anaesthetized) GAERS at different developmental stages (P15, P30 & P90)
  • Acquisition and analysis of multi-scale electrophysiological signals, with a special emphasis on cell excitability and network dynamics
  • In vivo intracellular or juxta-cellular labelling of cortical neurons, perfusion, histological processing and morphometric 3D reconstruction of neuronal elements (with the help of a histology technician)
  • In vitro patch-clamp recordings from somatosensory cortex slices to search for post-natal changes in voltage-gated channels and synaptic transmission
  • Writing of scientific publications
  • Supervision of a PhD student
Qualification & experience required
  • PhD in Neurosciences
  • Animal training courses
  • The candidate must have at least 5-year experience (including PhD researches) in an electrophysiology laboratory
Profile, skills & ability required
  •  Expert in cellular and network physiology
  •  Deep knowledge in electronics
  •  Deep knowledge in animal physiology and anesthesiology
  •  Surgery on small animals and in vivo intracellular or patch-clamp recordings
  •  Knowledge of analysis softwares (Spike2, Matlab)
  •  Proficiency in written and spoken English
  •  Autonomy, sense of initiative and contact
Application Process Candidates should submit curriculum vitae, including a list of publications, a description of their past and present research activity, their technical skills and contact information for two referees. Application should be sent to stephane.charpier@upmc.fr and severine.mahon@upmc.fr

Contact: Stéphane Charpier       Contact: Séverine Mahon

Intracerebral recordings, microelectrodes, epilepsy, spike sorting

 

DURATION 2 years (possible renewal)
STARTING DATE 01/11/2017
BRIEF DESCRIPTION OF THE PROJECT 

Single unit and LFP analysis in human epileptic patients

The general goal of this project is to improve knowledge on pathophysiology of epilepsy, in particular the interictal events and the epileptic seizures. With the development of intracerebral recordings with microelectrodes in our epileptic patients, we are now able since several years to perform continuous intracerebral recordings at different scales, from usual macroelectrodes to single neuron recordings, in the interictal period but also during seizures, in multiple intracerebral areas.

The main role of the position will be to perform electrophysiological analysis of intracerebral signals at different periods: during the interictal period and preictal period and to study the link with multiunit/single unit activities in cortical areas.

Main activities The doc /post-doc will be integrated in a multidisciplinary team including researchers, engineers, clinical electrophysiologists, neuroradiologists and neurosurgeons. He/she will receive a full assistance from an engineer for the data acquisition and storage. In addition, he/she will have inputs from several researchers/methodologists in signal analysis.
Qualification & experience required The candidate should have a PhD or equivalent in Signal analysis or Neuroscience related field. Expertise in signal processing and good analytical skills (MATLAB) are required, notably in spike sorting analysis. Since the analytic approaches needed for this study have been extensively developed and used in animals, previous experience in animal in vivo or in vitro experiments is a plus.
Application Process Candidates should submit curriculum vitae, a cover letter and contact information for two referees. Application should be sent to Pr Vincent Navarro
 (vincent.navarro@aphp.fr).

Contact: Vincent Navarro

PhD positions

Neurophysiology, epilepsy, translational reseach

 

DURATION 3 years
STARTING DATE 2018-2019
BRIEF DESCRIPTION OF THE PROJECT 

Multi-scale in vivo electrophysiology and morphology in a rodent genetic model of absence epilepsy

Absence epilepsy is a prototypic form of idiopathic generalized epilepsies whose epileptogenic processes remain largely unknown. The proposed postdoctoral work will consist in exploring in the GAERS, a validated model of the human pathology, the post-natal mechanisms responsible for the building-up of epileptic networks. The intake team recently showed that epileptic discharges first appear in the somatosensory cortex at the beginning of the third post-natal week and progressively evolved, in a three-step maturation process, into full-blown spike-and-wave discharges.

The goal of these doctoral researches is to establish causality links between the maturation of epileptic discharges and the post-natal developmental changes in the intrinsic excitability, synaptic function and morphology of the cortical neurons (and associated networks) responsible for the initiation of seizures.

Main activities
  • Combined in vivo EEG and intracellular (or patch-clamp) recordings from somatosensory cortex neurons in awake (or anaesthetized) GAERS at different developmental stages (P15, P30 & P90)
  • Acquisition and analysis of multi-scale electrophysiological signals, with a special emphasis on cell excitability and network dynamics
  • In vivo intracellular or juxta-cellular labelling of cortical neurons, perfusion, histological processing and morphometric 3D reconstruction of neuronal elements (with the help of a histology technician)
  • In vitro patch-clamp recordings from somatosensory cortex slices to search for post-natal changes in voltage-gated channels and synaptic transmission
  • Writing of scientific publications
Qualification & experience required
  • Masters degree in Neurosciences and/or related fields
  • Animal training courses
  • The candidate must have a  clear understanding of electrophysiology principles and methods.
Profile, skills & ability required
  •  Understanding of cellular and network physiology
  •  Knowledge in electronics
  •  Knowledge in animal physiology and anesthesiology
  •  Surgery on small animals and in vivo electrophysiology
  •  Knowledge of analysis softwares (Spike2, Matlab)
  •  Proficiency in written and spoken English
  •  Autonomy, sense of initiative and contact
Application Process Candidates should submit curriculum vitae, including a description of their past and present research activity, their technical skills and contact information for two referees. Application should be sent to stephane.charpier@upmc.fr and severine.mahon@upmc.fr

Contact: Stéphane Charpier       Contact: Séverine Mahon

Undergrad intern positions

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The lab is quite full at the moment, but you can always contact us for tips on other labs.